Coding

Part:BBa_K2686001:Design

Designed by: Thomas Jordan   Group: iGEM18_EPFL   (2018-09-30)


Encapsulin protein


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 77
    Illegal BglII site found at 441
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal SapI.rc site found at 375
    Illegal SapI.rc site found at 406


Design Notes

The part differs by three amino acids from the encapsulin already found on the registry, part BBa_K192000.

Source

This part directly stems from a plasmid encoding Thermotoga maritima encapsulin which was given to us by our hosting lab, and was originally a gift from David Savage's lab (Cassidy-Amstutz et al., 2016, Addgene plasmid #86405).

References

Cassidy-Amstutz, C., Oltrogge, L., Going, C., Lee, A., Teng, P., Quintanilla, D., East-Seletsky, A., Williams, E. and Savage, D. (2016). Identification of a Minimal Peptide Tag for in Vivo and in Vitro Loading of Encapsulin. Biochemistry, 55(24), pp.3461-3468.